LymphedemaV1, Main, Exploration, bibRecord, 00AA16

Evaluation of recombinant chitinase antigen in serological diagnosis and surveillance of lymphatic filariasis.

Identifieur interne : 00AA16 ( Main/Exploration ); précédent : 00AA15; suivant : 00AA17

Evaluation of recombinant chitinase antigen in serological diagnosis and surveillance of lymphatic filariasis.

Auteurs : S. Wang [République populaire de Chine] ; M. Xiong ; T. Liu ; L. Tang

Source :

The Southeast Asian journal of tropical medicine and public health [ 0125-1562 ] ; 1999.

RBID : pubmed:10774671

Descripteurs français

KwdFr :
- Animaux, Antigènes d'helminthe (immunologie), Brugia malayi (enzymologie), Brugia malayi (immunologie), Chitinase (immunologie), Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Filariose lymphatique (sang), Gerbillinae, Humains, Protéines de fusion recombinantes (immunologie), Technique de Western, Test ELISA (), Études d'évaluation comme sujet.
MESH :
- diagnostic : Filariose lymphatique.
- enzymologie : Brugia malayi.
- immunologie : Antigènes d'helminthe, Brugia malayi, Chitinase, Filariose lymphatique, Protéines de fusion recombinantes.
- parasitologie : Filariose lymphatique.
- sang : Filariose lymphatique.
- Animaux, Gerbillinae, Humains, Technique de Western, Test ELISA, Études d'évaluation comme sujet.

English descriptors

KwdEn :
- Animals, Antigens, Helminth (immunology), Blotting, Western, Brugia malayi (enzymology), Brugia malayi (immunology), Chitinases (immunology), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay (methods), Evaluation Studies as Topic, Gerbillinae, Humans, Recombinant Fusion Proteins (immunology).
MESH :
- chemical , immunology : Antigens, Helminth, Chitinases, Recombinant Fusion Proteins.
- blood : Elephantiasis, Filarial.
- diagnosis : Elephantiasis, Filarial.
- enzymology : Brugia malayi.
- immunology : Brugia malayi, Elephantiasis, Filarial.
- methods : Enzyme-Linked Immunosorbent Assay.
- parasitology : Elephantiasis, Filarial.
- Animals, Blotting, Western, Evaluation Studies as Topic, Gerbillinae, Humans.

Abstract

Apply recombinant chitinase fusion protein antigen, enzyme-linked immunosorbent assays examined anti-filarial antibodies and evaluated of useful value in serological diagnosis and surveillance of lymphatic filariasis. The test jirds were immunized and infected by chitinase and B. malayi third stage larvae respectively. Functional protein molecular of chitinase was analyzed by SDS-PAGE, Western blot. The result shown that jirds from microfilaremia (mf) and donors with Mf were directly to react with chitinase antigen that positive rate was 100%, but Mf-xt antigen was only 80%. Normal jirds and persons sera from unepidemic control donors all were negative. False positives of 5% and 20% reacted with chitinase and Mf-xt antigens respectively. The results indicate that recombinant chitinase antigen is suitable for detection of active occult or patent lymphatic filariasis with daytime blood samples in residents of endemic areas, is easy to be performed and inexpensive.

PubMed: 10774671

Affiliations:

République populaire de Chine

Le document en format XML

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<term>Brugia malayi (immunology)</term>
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<term>Elephantiasis, Filarial (blood)</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Elephantiasis, Filarial (parasitology)</term>
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<term>Antigènes d'helminthe (immunologie)</term>
<term>Brugia malayi (enzymologie)</term>
<term>Brugia malayi (immunologie)</term>
<term>Chitinase (immunologie)</term>
<term>Filariose lymphatique (diagnostic)</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Filariose lymphatique (parasitologie)</term>
<term>Filariose lymphatique (sang)</term>
<term>Gerbillinae</term>
<term>Humains</term>
<term>Protéines de fusion recombinantes (immunologie)</term>
<term>Technique de Western</term>
<term>Test ELISA ()</term>
<term>Études d'évaluation comme sujet</term>
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<term>Protéines de fusion recombinantes</term>
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<term>Blotting, Western</term>
<term>Evaluation Studies as Topic</term>
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<term>Humans</term>
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<term>Gerbillinae</term>
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<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Études d'évaluation comme sujet</term>
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<front><div type="abstract" xml:lang="en">Apply recombinant chitinase fusion protein antigen, enzyme-linked immunosorbent assays examined anti-filarial antibodies and evaluated of useful value in serological diagnosis and surveillance of lymphatic filariasis. The test jirds were immunized and infected by chitinase and B. malayi third stage larvae respectively. Functional protein molecular of chitinase was analyzed by SDS-PAGE, Western blot. The result shown that jirds from microfilaremia (mf) and donors with Mf were directly to react with chitinase antigen that positive rate was 100%, but Mf-xt antigen was only 80%. Normal jirds and persons sera from unepidemic control donors all were negative. False positives of 5% and 20% reacted with chitinase and Mf-xt antigens respectively. The results indicate that recombinant chitinase antigen is suitable for detection of active occult or patent lymphatic filariasis with daytime blood samples in residents of endemic areas, is easy to be performed and inexpensive.</div>
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<name sortKey="Xiong, M" sort="Xiong, M" uniqKey="Xiong M" first="M" last="Xiong">M. Xiong</name>
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Serveur d'exploration sur le lymphœdème

Evaluation of recombinant chitinase antigen in serological diagnosis and surveillance of lymphatic filariasis.

Evaluation of recombinant chitinase antigen in serological diagnosis and surveillance of lymphatic filariasis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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